PhD-Industrial Biotechnology- Austrian Centre of Industrial Biotechnology, Austria (Graz)
Location: Graz, Austria
Description: Austria has a long tradition in industrial biotechnology – particularly Vienna and Graz have established themselves as Centres of Biotech Research. Graz University of Technology and the University for Natural Resources and Life Sciences in Vienna are well-known for their work in industrial biotechnology. In Graz the efforts led to the formation of the Research Centre Applied Biocatalysis (A-B), in Vienna the Austrian Center of Biopharmaceutical Biotechnology (ACBT) was founded as a pioneering research organization.
ACIB is dedicated to a well balanced gender ratio. Therefore this job advertisement addresses qualified male and female persons equally. ACIB is not part of any collective agreement under Austrian law. It is intended to offer a minimum yearly gross payment of EUR 29.200,– for the PhD position advertised.
Chinese hamster ovary (CHO) cells are the primary workhorses for the production of therapeutic proteins. While the optimization of protein production via the modification of process parameters is well established, potential optimization targets within the cells themselves so far received less attention. Based on the recently available genome sequence we reconstructed the global metabolic pathways. To validate the mathematical model you will aim to quantitatively characterize CHO metabolism and its impact on biomass composition. You will run multiple fermentations of wild type and producer cell lines under different experimental conditions, such as media with and without glutamine, and assess their differences in terms of their metabolic flux profiles. The work will be carried out in tight collaboration with a leading industrial partner. Based on this data a parallel project will identify metabolic bottlenecks or engineer targets by CRISPR technology.
The successful applicant has a master’s degree in biotechnology, biochemistry/molecular biology or similar with previous experience in running fermentations of microbial or mammalian cells. A strong interest in working quantitatively is absolutely essential. Hands-on experience with analysis of mass spectrometry data (e.g. analysis of medium composition) is of advantage. Strong skills in written and spoken English are expected, as well as the ability to work both independently and as a member of a team.
How to apply:
Please send your application in pdf format to firstname.lastname@example.org indicating the reference number 04_2016 in the subject of the email.
Academic supervision by Ao.Univ. Prof. Dipl.-Ing. Dr.nat.techn. Nicole Borth, further information may be obtained from email@example.com.